Professor of Cell Biology

Manager of Cell Biology Core Facility

Contact Information

Haixia Institute of Science and Technology (HIST)
Fujian Agriculture and Forestry University (FAFU)

15 Shang xia dian Road

Fuzhou, Fujian 350002, P. R. China

Phone: 86-158-21752269

Email: shinnagawa@hotmail.com

Education

2004-2007,Ph.D., The University of Tokyo, Japan

2004-2007, M.S., The University of Tokyo, Japan

1998-2002, B.S., The University of Tokyo, Japan

Professional Experience

2017-Present, Professor/Manager of Cell Biology Core Facility,Fujian Agriculture and Forestry University, China

2012-2017, Support Principal Investigator/Manager of Cell Biology Core Facility, Shanghai Center for Plant Stress Biology, China

2008-2012, Postdoctoral Researcher, University of California, Riverside, US

2007-2008, Postdoctoral Researcher, The University of Tokyo, Japan

Research Interests

Development of a multicellular organism is a complicated process that requires both developmental/environmental signal perception and proper spatiotemporal regulation of many cellular processes involved in the differentiation of diverse cell types.  Some of these key cellular processes include endomembrane trafficking and the cytoskeleton. However, our knowledge of the regulation of these cellular processes and their linkage to developmental patterns is scarce.  My research interest is to reveal mechanisms underpinning the coordination of such cellular activities and their linkage to the development of specific tissues containing multiple cell types.  Besides interests in biological questions mentioned above, I am also interested in developing new tools for visualizing dynamic biological processes. Advances in innovative imaging tools such as novel molecular markers along with the acquisition of the state of the art microscopes in the cell biology core facility will generate unprecedented research opportunities for biologists. Our research projects are summarized below.

Publications

Liu X, Shi J, Fan H, Jiao J, Gao L, Tan L, Nagawa S, and Wang D, Nuclear DNA is Replicated during Zygote Development in Arabidopsis thaliana and Torenia fournieri Plant Physiol in press

Wang L, Cheng M, Yang Q, Li J, Wang X, Zhou Q, Nagawa S, Xia B, Xu T, Huang R, He J, Li C, Fu Y, Liu Y, Bao J, Wei H, Li H, Tan L, Gu Z, Xia A, Huang X, Yang Z, Deng XW. Arabinogalactan protein-rare earth element complexes activate plant endocytosis. Proc Natl Acad Sci U S A. 2019 Jul 9;116(28):14349-14357.

Wang Q, Li Y, Ishikawa K, Kosami KI, Uno K, Nagawa S, Tan L, Du J, Shimamoto K, Kawano Y. Resistance protein Pit interacts with the GEF OsSPK1 to activate OsRac1 and trigger rice immunity. Proc Natl Acad Sci U S A. 2018 Dec 4;115(49):E11551-E11560.

Wong HL, Akamatsu A, Wang Q, Higuchi M, Matsuda T, Okuda J, Kosami KI, Inada N, Kawasaki T, Kaneko-Kawano T, Nagawa S, Tan L, Kawano Y, Shimamoto K. In vivo monitoring of plant small GTPase activation using a Förster resonance energy transfer biosensor. Plant Methods. 2018 Jul 7;14:56

Gao L, Guo X, Liu XQ, Zhang L, Huang J, Tan L, Lin Z, Nagawa S, Wang DY. Changes in mitochondrial DNA levels during early embryogenesis in Torenia fournieri and Arabidopsis thaliana. Plant J. 2018 Jun 8. doi: 10.1111/tpj.1398

Rosas-Diaz T, Zhang D, Fan P, Wang L, Ding X, Jiang Y, Jimenez-Gongora T, Medina-Puche L, Zhao X, Feng Z, Zhang G, Liu X, Bejarano ER, Tan L, Zhang H, Zhu JK, Xing W, Faulkner C, Nagawa S, Lozano-Duran R. A virus-targeted plant receptor-like kinase promotes cell-to-cell spread of RNAi. Proc Natl Acad Sci U S A. 2018 Feb 6;115(6):1388-1393.

PubMed PMID: 29363594; PubMed Central PMCID: PMC5819414.

Pan J, Yuan H, Zhang X, Zhang H, Xu Q, Zhou Y, Tan L, Nagawa S, Huang ZX, Tan X. Probing the Molecular Mechanism of Human Soluble Guanylate Cyclase Activation by NO in vitro and in vivo. Sci Rep. 2017 Feb 23;7:43112.

Xu T, Dai N, Chen J, Nagawa S, Cao M, Li H, Zhou Z, Chen X, De Rycke R, Rakusová H, Wang W, Jones AM, Friml J, Patterson SE, Bleecker AB, Yang Z. Cell surface ABP1-TMK auxin-sensing complex activates ROP GTPase signaling. Science. 2014 Feb 28;343(6174):1025-8.

Lin D*, Nagawa S*, Chen J, Cao L, Chen X, Xu T, Li H, Dhonukshe P, Yamamuro C, Friml J, Scheres B, Fu Y, Yang Z. A ROP GTPase-dependent auxin signaling pathway regulates the subcellular distribution of PIN2 in Arabidopsis roots. Curr Biol. 2012 Jul 24;22(14):1319-25. * These authors contributed equally.

Nagawa S*, Xu T*, Lin D, Dhonukshe P, Zhang X, Friml J, Scheres B, Fu Y, Yang Z. ROP GTPase-dependent actin microfilaments promote PIN1 polarization by localized inhibition of clathrin-dependent endocytosis. PLoS Biol. 2012;10(4):e1001299. * These authors contributed equally.

Drakakaki G, Robert S, Szatmari AM, Brown MQ, Nagawa S, Van Damme D, Leonard M, Yang Z, Girke T, Schmid SL, Russinova E, Friml J, Raikhel NV, Hicks GR. Clusters of bioactive compounds target dynamic endomembrane networks in vivo. Proc Natl Acad Sci U S A. 2011 Oct 25;108(43):17850-5.

Li H, Lin D, Dhonukshe P, Nagawa S, Chen D, Friml J, Scheres B, Guo H, Yang Z. Phosphorylation switch modulates the interdigitated pattern of PIN1 localization and cell expansion in Arabidopsis leaf epidermis. Cell Res. 2011 Jun;21(6):970-8.

Kinoshita A, Betsuyaku S, Osakabe Y, Mizuno S, Nagawa S, Stahl Y, Simon R, Yamaguchi-Shinozaki K, Fukuda H, Sawa S. RPK2 is an essential receptor-like kinase that transmits the CLV3 signal in Arabidopsis. Development. 2010 Nov;137(22):3911-20.

Xu T, Wen M, Nagawa S, Fu Y, Chen JG, Wu MJ, Perrot-Rechenmann C, Friml J, Jones AM, Yang Z. Cell surface- and rho GTPase-based auxin signaling controls cellular interdigitation in Arabidopsis. Cell. 2010 Oct 1;143(1):99-110.

Kuroha T, Tokunaga H, Kojima M, Ueda N, Ishida T, Nagawa S, Fukuda H, Sugimoto K, Sakakibara H. Functional analyses of LONELY GUY cytokinin-activating enzymes reveal the importance of the direct activation pathway in Arabidopsis. Plant Cell. 2009 Oct;21(10):3152-69.

Nagawa S, Sawa S, Sato S, Kato T, Tabata S, Fukuda H. Gene trapping in Arabidopsis reveals genes involved in vascular development. Plant Cell Physiol. 2006 Oct;47(10):1394-405.