Shikui Song
Associate Professor of Plant Transformation
Manager of Plant Transformation Core Facility
Contact Information
Haixia Institute of Science and Technology, Fujian Agriculture and Forestry University, No. 15 Shangxiadian Road, Fuzhou, Fujian, China
Email: shikui.song@fafu.edu.cn
Education
2011-2015, Postdoc Fellow, Chinese Academy of Agricultural Sciences, China
2003-2008, Ph.D., University of Northeast Agricultural University, China
2000-2003, M.S., University of Northeast Agricultural University, China.
1989-1991, Life Science of Linyi Normal College, China
Professional Experience
2015-Present, Associate professor and Manager of Plant Transformation Core Facility, Fujian Agriculture and Forestry University, China.
2008-2015, Research scientist, Syngenta Biotechnology Ltd, China
1991-2000, Teacher, Zhaoxian Junior Middle School, China
Research Interests
The most convenient technique for introduce genes into plants is by Agrobacterium and biolistic. My focus is on Agrobacterium-mediated plant transformation. The major projects in my group:
- Plants transformation including tea tree, quinoa, soybean, strawberry and rice.
- Quninoa mutant library construction and candidate gene identification.
We have established soybean and rice transformation protocols with high TF, and provided soybean transformation service for many universities and research institutes. We also got good results on tea tree, strawberry and quinoa transformation. We have got transgenic buds of tea tree, quinoa and strawberry. And two efficient transformation systems in quinoa have been established.
From left to right: GUS assay of quinoa, GUS assay of tea buds
From left to right: Quinoa hairy root system, Quninoa mutant screening
Publications
Jaikishun S., Gang P., Song S., Yang Z., (2020) Morpho-physiological responses of quinoa varieties to salinity in a hydroponic system. American Journal of plant physiology. 15(1):41-51
Bai, M; Yuan, J; Kuang, H; Gong, P; Li, S; Zhang, Z; Liu, B; Sun, J; Yang, M; Yang, L; Wang, D; Song, S; Guan, Y (2020) Generation of a multiplex mutagenesis population via pooled CRISPR‐Cas9 in soya bean. Plant biotechnology Journal, 18(3):721-731
Wang J; Kuang H; Zhang; Yang; Yan L; Zhang M; Song S; Guan Y. (2020) Generation of seed l ipoxygenase-free soybean using CRISPR-Cas9. The Crop Journal, 8(3):432-439.
Jaikishun, S. Li W., Yang Z., Song, S. (2019) Quinoa: In perspective of global challenges. Agronomy, 9(4): 176.
Yu Y,Hou W,Yael H,Sun S,Wu C,Ifat M,Song S, Rachel M, Han T (2018) Constitutive expression of feedback-insensitive cystathionine gamma-synthase increases methionine levels in soybean leaves and seeds. BMC Plant Biology, 17(1):54-62.
Song S, Wang Y, Yang Y, Geng L, Zhang C, Li X, Han T. (2014)CRISPR/Cas-based Genome Editing in Crops. Molecular Breeding, 12(6):1059-1066 (In Chinese).
Song S., Hou W., Godo I., Wu C., Yu Y., Matityahu I., Hacham Y., Sun S., Han T., Amir R. (2013) Soybean seeds expressing feedback-insensitive cystathionine r-synthase exhibit a higher content of methionine., Journal of Experimental Botany, 64 (7): 1917-1926
Cao D; Hou W; Song S; Sun H; Wu C; Gao Y; Han T. (2009) Assessment of conditions affecting Agrobacterium rhizogenes-mediated transformation of soybean. Plant Cell, Tissue and Organ Culture, 96(1):45-52.
Jian, B; Hou, W; Wu, C, Liu, B; Liu, W; Song, S; Bi, Y; Han, T (2009) Agrobacterium rhizogenes-mediated transformation of Superroot-derived Lotus corniculatus plants: a valuable tool for functional genomics. BMC Plant Biology, 9:78—91.