Manager:

Xiaomin Yu (xmyu0616@fafu.edu.cn)

Maintenance Staff:

Xiaxia Wang

Zhenghua Du

Ruimei Wu

Phone:

(86)59183855671

Core Location:

Building Name: Haixia Institute for Science and Technology

Room Number: 606

Core Summary:

The Metabolomics Facility was established in 2015. It is a mass spectrometry-based facility for analyses of small molecule metabolites in a wide variety of biological systems. The Metabolomics facility currently houses two electrospray ionization mass spectrometers and two gas chromatography-mass spectrometers. We offer fee-based services to the FAFU research community and off-campus researchers. Services include initial consultation, method development, data acquisition, interpretation and presentation. Key competencies include both targeted analyses of many compound classes as well as untargeted metabolomics to uncover potential biomarkers.

List of Instruments:

2 LCMS Instruments:

SYNAPT HDMS G2-Si Mass Spectrometer with I-class UPLC

SYNAPT G2-Si mass spectrometer provides the most uncompromising high resolution UPLC-MS/MS performance, with the greatest versatility and upgrade option to access 3 dimensions of resolution for the ultimate in discovery power. It combines StepWave, UPLC separations with Quantitative Time-of-Flight (QuanTof) technologies, for the highest peak capacity and data quality. StepWave is the most sensitive and robust ion source available through its unique ability to 'actively' select ions and 'passively' reject neutral contaminants. QuanTof provides high mass resolution, accurate mass and isotope ratios and wide dynamic range. The dual collision cell arrangement of TriWave enhances MS/MS possibilities, by providing Collision Induced Dissociation (CID) and/or Electron Transfer Dissociation (ETD) fragmentation with high resolution and accurate mass measurements. 

XEVO TQ-S Mass Spectrometer with I-class UPLC

XEVO TQ-S is an advanced benchtop tandem quadrupole mass spectrometer, designed to meet the needs of the most demanding quantitative LC/MS/MS applications. ScanWave technology allows ions within the collision cell to be accumulated and then separated according to their mass-to-charge ratio (m/z). Synchronizing the release of these ions with the scanning of the second quadrupole mass analyzer significantly enhances the signal intensity of full scan product ion spectra. Specifically designed to maximize sensitivity in all data acquisition modes, XEVO TQ-S is equipped with a larger ion sampling orifice, an enhanced vacuum pumping configuration and revolutionary StepWave ion transfer optics. With its sensitivity, we can detect target compounds in complex samples at very low concentrations, dilute samples to reduce matrix effects and work with smaller sample volumes.

2 GCMS instruments:

Pegasus HT GC-TOF-MS with Gerstel Multipurpose Sampler

Pegasus GC-TOFMS combines LECO’s Time-of-Flight Mass Spectrometer (TOFMS) with ChromaTOF software to provide revolutionary time-compressed chromatography, complex sample resolution, automated peak find and true signal deconvolution in a high throughput GCMS. It is capable of collecting 500 full-range mass spectra/second. The peak find algorithm automatically locates all peaks in the chromatogram, when provided with only an expected chromatographic peak width and a signal-to-noise threshold. Coeluting analyses, peaks buried beneath the background of the TIC and even small peaks buried beneath large matrix interferences are automatically detected and reported. After the peak find algorithm has located all of the analyses, a mass spectrum for each analyte is automatically extracted, free of interferences from the system background, matrix background and coeluting analyses using true signal deconvolution.

MDGC/GC-MS

This instrument is mainly composed of multi-dimensional GCs (MDGC), a mass spectrometer and a rotary pump. The core unit is a switching device. When the switching device is not used, it works as a conventional standalone GC; on the other hand, when the switching device is in use, it upgrades to MDGC. The competency of MDGC is its prominent separation ability. In normal GC analysis, the sample is introduced into a column where it is separated and eluted, and the resulting components are detected by a detector. However, MDGC is a technology for improving resolution beyond that of the regular GC analysis as it re-introduces the dissolved component into the second column.

This instrument enables targeted analysis of both simple and complex matrices (e.g., petroleum, flavor materials, environmental contaminants etc.).