Manager:

Xiaoyue Zhu, Ph.D. (hbmc_fcgc@163.com)

Core Location:

Haixia Institute for Science and Technology Room 609

Fujian Agriculture and Forestry University

15 Shangxiadian Road

Fuzhou, Fujian Province, P. R. China, 350002

Core Summary:

The Functional Chemical Genomics Facility houses a chemical library of plant natural compounds and a Tecan Freedom EVO200 multi-functional robotic workstation for high throughput screening. The facility also contains equipment necessary for routine mammalian cell culture. It provides fee-based screening services for the FAFU and other researchers.  The facility is also capable of designing and fabricating microfluidic devices and is open to collaboration on the development of these devices for the needs by biologists.

List of Instruments:

Tecan Freedom EVO® 200 workstation:

The Tecan Freedom EVO^® workstation is a flexible robotic platform for high throughput applications. The robot performs fully automated liquid-handling tasks with two pipetting arms and a manipulator arm. The workstation is integrated with the following peripheral devices:

• Te-ShakeÔ orbital shaker: This highly effective shaker could handle microplates, deep-well plates, half-skirted PCR plates, and microcentrifuge tubes. The shaker offers full software control of shaking parameters satisfying the needs of both rigorous mixing and delicate resuspension.
• MIOÔ monitored incubation option: This unit provides temperature-controlled incubation directly on the workdeck.
• HydrospeedÔ Plate washer: This microplate washer precisely controls the speed and power of aspiration and dispensing for cell, bead and ELISA washing applications.
• Spark^â microplate reader: This spectrophotometer is configured to support multiple detection modes including absorbance, fluorescence (top & bottom), time resolved fluorescence, fluorescence polarization, and luminescence.
• CytomatÔ 2C incubator: This CO₂ plate incubator accommodates all standard microplate formats under various temperatures, humidity ranges, and gaseous atmospheres.
• Laminar flow cabinet: HEPA Class 100 laminar air flow cabinet maintains a constant positive air pressure to prevent the intrusion of contamination.

Mammalian Cell Culture Equipment:

• Class 100,000 cleanroom suite
• Thermofisher ClassII Type A2 biological safety cabinet
• Thermofisher 4100 water-jacketed CO₂ incubator
• Thermofisher STERI-CYCLE i250 oxygen control (tri-gas) incubator
• Millipore Scepter 2.0 handheld automated cell counter
• Nikon Ts2-FL inverted fluorescence microscope
• TokaiHit TPi-TS2X ThermoPlate
• Thermofisher Locator JR liquid nitrogen tank
• Balance
• Centrifuge
• Constant temperature shaking water bath